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A DNA solution that is 800 ng/uL, and you want to use 0.01mg of DNA in a reaction. How many microliters of your DNA solution should you use?

 Sep 3, 2020
 #1
avatar+166 
+1

Hello. This is a difficult mixture problem. The following link directs you to a SIMILAR problem. I how it offers a general idea:

 

https://www.google.com/url?sa=t&source=web&rct=j&url=https://www.researchgate.net/post/Can_someone_help_me_with_a_conversion_dilution_calculation&ved=2ahUKEwjSoa6OlczrAhUkYTUKHXTQB2gQFjAAegQIAhAB&usg=AOvVaw1JZ3RHX3IkseHPt9jiKcLk

 Sep 3, 2020
 #2
avatar+10147 
+2

A DNA solution that is 800 ng/uL, and you want to use 0.01mg of DNA in a reaction. How many microliters of your DNA solution should you use?

 

Eine DNA-Lösung mit 800 ng / ul, und Sie möchten 0,01 mg DNA in einer Reaktion verwenden. Wie viele Mikroliter Ihrer DNA-Lösung sollten Sie verwenden?

 

Hello Guest!

 

\(\frac{800ngDNA}{\mu l}\cdot\frac{10^3mg}{10^9ng}=\frac{8\cdot 10^{-4}mg\ DNA}{\mu l}\)

 

\(0.01mg\ DNA:\frac{8\cdot 10^{-4}mg\ DNA}{\mu l}=\color{blue}12.5\ \mu l\)

 

\(12.5\ microliters\ of\ your\ DNA\ solution\ should\ you\ use. \)

laugh  !

 Sep 3, 2020
edited by asinus  Sep 3, 2020
 #3
avatar+27001 
+2

.01 mg  = 10 microgram = 10 000 nanograms

 

10 000 nanograms  /  800 nanograms/microliter = 12.5 microliters

 Sep 3, 2020

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