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Genetics

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A DNA solution that is 800 ng/uL, and you want to use 0.01mg of DNA in a reaction. How many microliters of your DNA solution should you use?

Sep 3, 2020

#1
+166
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Hello. This is a difficult mixture problem. The following link directs you to a SIMILAR problem. I how it offers a general idea:

Sep 3, 2020
#2
+10147
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A DNA solution that is 800 ng/uL, and you want to use 0.01mg of DNA in a reaction. How many microliters of your DNA solution should you use?

Eine DNA-Lösung mit 800 ng / ul, und Sie möchten 0,01 mg DNA in einer Reaktion verwenden. Wie viele Mikroliter Ihrer DNA-Lösung sollten Sie verwenden?

Hello Guest!

$$\frac{800ngDNA}{\mu l}\cdot\frac{10^3mg}{10^9ng}=\frac{8\cdot 10^{-4}mg\ DNA}{\mu l}$$

$$0.01mg\ DNA:\frac{8\cdot 10^{-4}mg\ DNA}{\mu l}=\color{blue}12.5\ \mu l$$

$$12.5\ microliters\ of\ your\ DNA\ solution\ should\ you\ use.$$

!

Sep 3, 2020
edited by asinus  Sep 3, 2020
#3
+27001
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.01 mg  = 10 microgram = 10 000 nanograms

10 000 nanograms  /  800 nanograms/microliter = 12.5 microliters

Sep 3, 2020